Leishmania Trypanothione Synthetase-Amidase Structure Reveals a Basis for Regulation of Conflicting Synthetic and Hydrolytic Activities
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Leishmania Trypanothione Synthetase-Amidase Structure Reveals a Basis for Regulation of Conflicting Synthetic and Hydrolytic Activities*S⃞
The bifunctional trypanothione synthetase-amidase catalyzes biosynthesis and hydrolysis of the glutathione-spermidine adduct trypanothione, the principal intracellular thiol-redox metabolite in parasitic trypanosomatids. These parasites are unique with regard to their reliance on trypanothione to determine intracellular thiol-redox balance in defense against oxidative and chemical stress and to...
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Trypanothione synthetase (TryS) is an important enzyme for survival of Leishmania; thus an important target for structure based drug design against leishmaniasis. We have constructed three-dimensional structure of the TryS of Leishmania infantum by homology modeling with acceptable Ramachandran statistics. The modeled structure TryS is compared with human (host) glutathione synthetase (GS) and ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2008
ISSN: 0021-9258
DOI: 10.1074/jbc.m801850200